ATPAF1 Knockout cell line (Hep G2)

ATPAF1 Knockout cell line (Hep G2) Order

Catalog#

YKO-HT7434

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ATPAF1 Knockout cell line (Hep G2)

Species

Human

Gene Name

ATPAF1

Gene ID

64756

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 2 (based on transcript ATPAF1-211)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATPAF1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATPAF1 gene knockout ATPAF1 Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ATPAF1 Knockout cell line (Hep G2)

Catalog#

YKO-HT7434

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Hepatoma Cell Line(Hep G2)
inquiry More details

Catalog#: YC-C001

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

ATPAF1 Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: ATPAF1

Gene ID: 64756

Size: 1*10^6

Catalog#: YKO-XN85753

YKO-XN85753(HeLa)
ATPAF1 Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: ATPAF1

Gene ID: 64756

Size: 1*10^6

Catalog#: YKO-XN85752

YKO-XN85752(HCT 116)
ATPAF1 Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: ATPAF1

Gene ID: 64756

Size: 1*10^6

Catalog#: YKO-XN85751

YKO-XN85751(HEK293)

Related Popular Products

Wild-type Cell Lines

Human Hepatoma Cell Line(Hep G2)

Price (USD): -

Catalog#: YC-C001

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

ATPAF1 Knockout cell line (HeLa)

Organism: Human

Gene Name: ATPAF1

Gene ID: 64756

Size: 1*10^6

Catalog#: YKO-XN85753

inquiry More details
ATPAF1 Knockout cell line (HCT 116)

Organism: Human

Gene Name: ATPAF1

Gene ID: 64756

Size: 1*10^6

Catalog#: YKO-XN85752

inquiry More details
ATPAF1 Knockout cell line (HEK293)

Organism: Human

Gene Name: ATPAF1

Gene ID: 64756

Size: 1*10^6

Catalog#: YKO-XN85751

inquiry More details

ATPAF1 Knockout cell line (Hep G2)

Ubigene